Hamza Belkhodja

University of Mustapha Stambouli

Title: Evaluation of antioxidant and hepatoprotective potential of Okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage

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Biography

Hamza BELKHODJA, born in 08/07/1989 in Sig, Mascara (Algeria), Married. After completing his Master degree in microbial biotechnology and health safety at the Department of Biology, University of Mustapha Stambouli, Mascara, Algeria, he achieved his Ph.D. degree in the Sciences, Technology and Health form the same University. He is currently a doctor at the University of Mascara, Department of Biology. His research interests are in the area of evaluation of potential of medicinal plants, as well as isolation, purification and characterization of bioactive compounds from plant sources. He has collaborated actively with researchers in several other disciplines of plants valorization. He has many research publications in National and International Journals.

Abstract

Oxidative stress is commonly defined as an imbalance between the generation of free radicals and the body's antioxidant defenses due to several factors such as UV rays, smoking, as well as toxic compounds. This can lead to the development of various pathologies (cancer, atherosclerosis, diabetes and liver damages). The objective of this work is to study the species Abelmoschus esculentus by the exploitation of bioactive components and the evaluation of the antioxidant activity in vitro and the study of the hepatoprotective potential in vivo against hepatic lesions induced by carbon tetrachloride (CCl4). The in vitro antioxidant activity of extracts from the plant Abelmoschus esculentus was evaluated by the DPPH free radical scavenging method. The quantitative determination reveals that the macerated aqueous extract of A.esculentus is the richest in bioactive components with contents of total polyphenols, flavonoids and condensed tannins equal to 85.59 ± 7.5 mg GAE / g, 71.54 ± 3.8 mg QE / g and 27.25 ± 0,35 mg CE / g respectively. Abelmoschus esculentus extracts show interesting free radical properties manifested by low IC50 values compared to the standard antioxidant (Ascorbic Acid). While the study of hepatoprotective activity in vivo shows that the macerated aqueous extract of Abelmoschus esculentus has an interesting hepatoprotective effect on the lesions induced by carbon tetrachloride in mice. It is manifested by weight gain, normal hepato-somatic index and improvement in biochemical parameters. The species of A. esculentus is therefore of considerable therapeutic interest as an alternative compound for the prevention against liver disorders and lesions.